January 31, 2020

A new technical paper, co-authored by M. Ronikier from our group, was published in PeerJ.

The analyses were led by A. Ronikier and P. Janik, the team working on nivicolous myxomycetes in the Institute of Botany. It describes a new DNA isolation/amplification protocol for myxomycetes, which allows to obtain amplicons of loci from small fractions of spores taken from a single sporophore. It thus allows to overcome the main limitation in the molecular studies of this specific group of protists, namely scarcity of the available biological material for a majority of taxa. Being nearly non-destructive, it can substantially improve taxon sampling while sequencing selected DNA fragments for barcoding and phylogeny. This approach also has other important advantages, in particular it gives a unique possibility to apply both molecular and morphological assays to the same structure (sporophore), which then can be further stored as documentation. The method has already been successfully applied further in a number of ongoing phylogenetic analyses.

Citation

Janik P, Ronikier M, Ronikier A, 2020. New protocol for successful isolation and amplification of DNA from exiguous fractions of specimens: a tool to overcome the basic obstacle in molecular analyses of myxomycetes. PeerJ 8: e8406. DOI